Table 2 Outcome of FPMSC isolation and expansion under GMP requirements
Sample ID | W455623000021 | W455623000032 | W455623000038 | W455623000040 | Average |
|---|---|---|---|---|---|
Donor age | 23 | 42 | 44 | 50 | 39.7 ± 11.7 |
Donor sex | Male | Female | Male | Female | |
Vol. starting material (ml) | 20 | 19.5 | 10 | 24 | 17 ± 3.82 |
Initial number of cell (x106) | 11 | 2.5 | 5.29 | 3.19 | 5.48 ± 3.83 |
Initial viability (%, > 70%) | 83 | 80 | 71 | 77 | 78 ± 0.05 |
Time until 1st passage (days) | 8 | 14 | 10 | 10 | 10.5 ± 2.52 |
Total cells at P1 (x107) | 1.43 | 1.20 | 1.23 | 1.24 | 1.28 ± 0.10 |
Passage viability (%, > 70%) | 99 | 89 | 88 | 96 | 93 ± 0.05 |
Total cell harvest (x108) | 1.55 | 1.05 | 1.00 | 1.01 | 1.15 ± 0.27 |
Harvest time (days) | 12 | 20 | 20 | 16 | 17 ± 3.83 |
Cell viability at final harvest (%) | 100 | 96 | 95 | 97 | 97 ± 0.02 |