Fig. 2

NAC ameliorates DEP-induced inflammation in RAW264.7 macrophages. (a) RAW264.7 macrophages were pretreated with CCXB (COX-2 inhibitor), INDO (COX inhibitor), or NAC (antioxidant) for 2 h, followed by DEP exposure. COX-2 protein levels were determined using western blot. The expression levels of the proteins were quantified using ImageJ. β-actin was used as the internal control. Data are presented as the mean ± standard deviation. *p < 0.05 and **p < 0.01 vs. vehicle control. Data were obtained from three independent experiments. (b) Viability of RAW264.7 macrophages exposed to CCXB, INDO, and NAC treatment. RAW264.7 macrophages were treated with 10 µM CCXB, 10 µM INDO, and 10 mM NAC for 48 h. NS, no significance