Fig. 5
From: Canine intestinal organoids as a platform for studying MHC class II expression in epithelial cells
The effect of IFN-γ on MHC class II protein expression in colonoids. Impact of IFN-γ exposure on DLA-DR molecule expression. (A) Representative confocal images of colonoids 48 h after IFN-γ treatment (0, 30 and 60 ng/mL) in DM. DLA-DR (green), nuclei (DAPI, blue) and F-actin (red) were stained. Scale bar = 5 µm. (B) Comparative analysis of mean intensity of DLA-DR at 48 h after IFN-γ treatment (0, 30 and 60 ng/mL) in DM. Fifteen fields of view were randomly selected from two biological replicates. Mean intensity was normalized using number of nuclei in the same image. The error bars represent the SEM. ***p< 0.001