Fig. 1

Differentiation of canine colonoids. (A) A schematic representation of the flow of the differentiation induction. The colored dots in the intestinal organoid represent specialized epithelial cells, like goblet and enteroendocrine cells, that differentiate from stem cells. The canine colonoids were initially cultured for 2 days in expansion medium (EM) followed by changing the medium from EM to differentiation medium (DM). For comparison group, colonoids cultured in EM total of 6 days were used. The colonoids were collected at day 2, 4 and 6 after passaging. This schematic was created with BioRender.com. (B) The representative image of the canine colonoids at day 2 and 6. Scale bar = 50 μm. (C) Three differentiation markers’, Chromogranin A (CgA), Lysozyme (Lyz) and Mucin 2 (MUC2), mRNA were quantified using RT-qPCR. Two biological replicates were used for this analysis and differentiation/non-differentiation treatment were replicated three times. The error bars represent the standard error of the mean (SEM). *p< 0.05, **p< 0.01