Fig. 3

Evaluating the function of Lipofectamine 2000 in different cell types and conditions: HEK293 cells and GFP-PEG plasmid (4 h and 24 h) (A), WJ-MSCs and GFP-PEG plasmid (4 h and 24 h) (B), and WJ-MSCs and mimic-Mmu-miR-210-3p-FAM (C). Evaluating the best conditions of miRNA-mimic complex (7 or 4 mimic-miRs complex) at different concentrations 100 pM (D) or 200 pM (E) using Lipofectamine 2000 and time points (after 48 h and 72 h). MSCs (mesenchymal stromal cells); Chem_ind (chemical induction); Diff_1 (differentiated cells after week 1); Diff_2 (differentiated cells after week 2); NC (non-coding miRNAs); The asymmetry in the error bars is due to the logarithmic scale used for the Y-axis; X-axis represents the different HLCs produced by various methods and The Y-axis is fold change of gene expression; the scale bars in Fig. 3A-C are equal to 100 μm