Fig. 4

Depletion of YAP/TAZ affects morusin-induced apoptosis. (A) Enhanced morusin-induced apoptosis due to YAP/TAZ depletion in a dose-dependent manner. HEK293A cells were transfected with either siNC or siYAP/siTAZ and treated with either DMSO or morusin for 6 h at the indicated concentrations. (B) Enhanced morusin-induced apoptosis due to YAP/TAZ depletion in a time-dependent manner. HeLa cells were transfected with either siNC or siYAP/siTAZ and treated with either DMSO or morusin (50 µM) at the indicated time points. (C) MINK1 modulates morusin-induced apoptosis in a dose-dependent manner. HCT116 cells overexpressing either wild-type (WT) or a constitutively active form (CA) of HA-MINK1 were treated with either DMSO or morusin for 6 h at the indicated concentrations. (D) MINK1 modulates morusin-induced apoptosis in a time-dependent manner. HCT116 cells overexpressing either wild-type, a constitutively active form of HA-MINK1 or YAP/TAZ-depleted HA-MINK1 CA were treated with either DMSO or morusin (50 µM) at the indicated time points. (E) Determination of cell survival using the MTT assay. HeLa cells were depleted of YAP/TAZ or overexpressed with MINK1 CA and treated with either DMSO or morusin for 4 h at the indicated concentrations. Cell viability was assessed by measuring the optical density of the MTT-formazan product. Statistical analysis was performed using one-way ANOVA followed by Bonferroni’s multiple comparison test, where ***p < 0.001, **p < 0.01. Uncropped full-length blots are presented in Supplementary Fig. 1