Fig. 3
From: Transient activation of YAP/TAZ confers resistance to morusin-induced apoptosis
Depletion of YAP/TAZ affects morusin-induced stress granule formation. (A) Reduced stress granule formation due to YAP/TAZ depletion. A549 and HeLa cells were transfected with either siNC or siYAP/siTAZ and treated with either DMSO or morusin (30 µM) for 3 h. Endogenous G3BP1 puncta were detected using immunostaining with an anti-G3BP1 antibody. Depletion of YAP and TAZ was confirmed by immunoblotting with anti-YAP/TAZ antibody. (B) Time-dependent effect of YAP/TAZ depletion on stress granule formation. HCT116 cells were transfected with either siNC or siYAP/siTAZ and treated with either DMSO or morusin (30 µM) at the indicated time points. Endogenous G3BP1 puncta were detected using immunostaining with an anti-G3BP1 antibody. (C) Quantitation of cells with G3BP1 puncta. The percentage of cells exhibiting G3BP1 puncta were quantified in three different arbitrary areas and is presented in the graph. Data are shown as the mean ± SEM. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparison test (***p < 0.001 compared to the indicated points; n = 3)