Table 1 Oligonucleotides for qPCR
Gene | Forward primer | Reverse primer |
|---|---|---|
B2M | TGCTGTCTCCATGTTTGATGTATCT | TCTCTGCTCCCCACCTCTAAGT |
Atoh1 | CCTTCCAGCAAACAGGTGAATGG | CTTTCCCACCTGCTTGCATT |
BMP2 | ATTCCCCGTGACCAGACTTT | CTTTCCCACCTGCTTGCATT |
BMP7 | CATGAGCTTCGTCAACCTCG | AAACCGGAACTCTCGATGGT |
Osteocalcin | CGCTACCTGTATCAATGGCTGG | CTCCTGAAAGCCGATGTGGTCA |
sFRP2 | ATAAAAATGATGATGACAACGACATA | ATCTCCTTCACTTTTATTTTCAGTG |
Axin2 | CGATGAGTTTGCCTGTGGAG | CCGTCTCATCCTCCCAGATC |
PDGF | CCAGCGACTCCTGGAGATAGA | CTTCTCGGGCACATGCTTAGT |
VEGF-A | GCACCCATGGCAGAAGG | CTCGATTGGATGGCAGTAGCT |
HIF1α | TATGAGCCAGAAGAACTTTTAGGC | CACCTCTTTTGGCAAGCATCCTG |
Osterix | TTCTGCGGCAAGAGGTTCACTC | GTGTTTGCTCAGGTGGTCGCTT |
Runx2 | CCCAGTATGAGAGTAGGTGTCC | GGGTAAGACTGGTCATAGGACC |
Dlx5 | TACCCAGCCAAAGCTTATGCCG | GCCATTCACCATTCTCACCTCG |
Collagen 1A1 | GATTCCCTGGACCTAAAGGTGC | AGCCTCTCCATCTTTGCCAGCA |
BSP | GGCAGTAGTGACTCATCCGAAG | GAAAGTGTGGTATTCTCAGCCTC |
DMP1 | GAGCAGTGAGTCATCAGAAGGC | GAGAAGCCACCAGCTAGCCTAT |
DSPP | CAACCATAGAGAAAGCAAACGCG | TTTCTGTTGCCACTGCTGGGAC |
OPN | CGAGGTGATAGTGTGGTTTATGG | GCACCATTCAACTCCTCGCTTTC |
ALP | ACCATTCCCACGTCTTCACA | AGGGCTTCTTGTCTGTGTCA |