Fig. 2

Ectopic expression of Atoh1 promotes odontogenic differentiation. DPSCs treated with Adv-Null and Adv-Atoh1 were maintained in the odontogenic medium. Total RNA was isolated for qPCR analysis. A Expression of Osterix on day 7 and 14 (n = 6). B Expression of Runx2 on day 4, 7, and 14 (n = 3 or 6). C DPSCs were fixed and stained with Alizarin Red on day 20 and photographed (top). Cell-bound Alizarin Red was extracted and quantified by light absorbance at 450 nm (bottom; n = 3)