Fig. 4

RAP2A is the target mRNA of miR-526b. A The binding sites between RAP2A and miR-526b were predicted by the ENCORI database. B-C Luciferase reporter assay and RNA pull-down assay were utilized to verify the interaction of RAP2A and miR-526b. D-E RT-PCR results of RAP2A expression in PTC tissues/cell lines compared to normal tissues/cell lines. F Pearson’s analysis was performed to show the correlation of RAP2A and miR-526b or circPSD3 in PTC samples. G Luciferase reporter assay was utilized for verifying the regulation of circPSD3 on the luciferase activity of RAP2A. All experiments were repeated at least three times and data is representative of three independent experiments (n = 3). **P < 0.01; ***P < 0.001