Fig. 4
From: Transcriptome and DNA methylation profiling during the NSN to SN transition in mouse oocytes
Overlap of SN differentially methylated regions with histone H3K4me3, H3K27me3 and H3K36me3 domains. A Genome screenshot showing the distribution of H3K4me3 (dark blue), H3K27me3 (yellow) and H3K36me3 (dark red) marks compared to input (grey) in GV oocytes. Data was quantified as RPKM for 2 kb running windows. Annotated genes and oocyte transcripts (forward in pink, reverse in green), DNA methylated (red) and unmethylated (blue) and CpG islands (grey) [64] are shown as annotation tracks above. H3K36me3 enrichment aligns well with methylated domains, as has been previously reported [51]. The grey box highlights a region with overlapping H3K27me3 and H3K36me3. B Pie chart showing the number of 2 kb windows (N) defined in each chromatin category, based on levels of H3K4me3, H3K27me3 and H3K36me3 in GV oocytes (Supplementary Figure S7C). C Barplot showing the percentage of histone-enriched 2 kb windows that fell within unmethylated, intermediate methylated or methylated domains (annotation from [39]). Chi-square statistical test (p < 0.00001). D The barplot shows the log2 fold enrichment for histone-marked 2 kb windows within SN hyper DMRs (N = 1,099) and Uhrf1 hypo DMRs (N = 23,387) compared to a random set of domains (N = 1,100) (Chi-Square statistical test, p < 0.0001 and p < 0.0001, respectively)