Fig. 3

DNA methylation changes during NSN to SN transition. A, B Violin plots showing average CpG (A) and Non-CpG methylation (B) in NSN, SN and MII oocytes. C Violin plots comparing DNA methylation (CpG methylation) between NSN and SN oocytes in oocyte unmethylated (N = 52,452), intermediate (N = 88,103) and methylated domains (N = 37,272) based on published annotations from GV oocytes [39]. Intermediate (or partially) methylated domains were defined as regions covered neither by unmethylated nor methylated domains. D Scatterplot of DNA methylation values (%) of NSN vs. SN oocytes. Quantified were unmethylated, intermediate and methylated domains. Each dot represents one domain. Differentially methylated regions (DMRs) are highlighted in red (hypermethylated in SN) or blue (hypomethylated in SN). E Enrichment analysis showing the odds ratio of SN hyper DMRs (red) or random domains (grey) overlapping intergenic regions, oocyte transcripts, promoters or domains hypomethylated in Uhrf1 KO GV oocytes. The number of domains overlapping domains with a feature is indicated. NSN and SN oocytes comprise a mixture of oocytes from the present study and oocytes from [40]. MII oocyte data is from [44]