Fig. 2

Development of a classifier to infer chromatin configuration in oocyte RNA-seq datasets. A Heatmap showing separate clustering of NSN and SN oocytes based on the 100 classifier genes in our NSN and SN dataset. Colour scale indicates relative expression (Z score) for each sample and classifier gene. Samples are colour-coded based on their chromatin configuration and genes are split into classifier genes that are down or upregulated in SN compared to NSN oocytes. B, D UMAP plots showing clustering based on the transcriptional profile of the 100 classifier genes of our NSN and SN oocytes together with B young (12 weeks) and old oocytes (> 40 weeks) from [40] and D Exosc10 control and KO oocytes from [41]. The separate clusters of our NSN and SN oocytes are encircled by a dotted line. Each point indicates a separate sample. The shape of the point indicates the origin of the sample (study), whereas the colour shows the sample condition. C Stacked bar charts showing the proportion of NSN and SN oocytes for each genotype, based on our transcriptome classification. Aged oocytes have proportionally more SN oocytes than young oocytes, whereas Exosc10 KO oocytes have proportionally fewer SN oocytes than Exosc10 control oocytes