Fig. 3
From: Mutant Fam20c knock-in mice recapitulate both lethal and non-lethal human Raine Syndrome
The skeletal characteristics of the conditional and conventional Fam20cD446N knock-in mice. (A, A’, A’’) Masson staining of the P4W WT femoral metaphysis (A), femur heads (A’) and femoral cortex (A’’). (B, B’, B’’) Masson staining of the P4W conditional Fam20cD446N knock-in femoral metaphysis (B), femur heads (B’) and cortex (B’’). (C, C’, C’’) Masson staining of the P4W conventional Fam20cD446N knock-in femoral metaphysis (C), femur heads (C’) and cortex (C’’). (D, D’, D’’) Immunohistochemical staining with antibody against Fam20C in the tibiae of the P4W WT (D), conditional (D’) and conventional Fam20cD446N knock-in mice (D’’). (E, E’, E’’) Immunohistochemical staining with antibody against Fgf23 in the tibiae of the P4W WT (E), conditional (E’) and conventional Fam20cD446N knock-in mice (E’’). (F, F’, F’’) Immunohistochemical staining with antibody against DMP1 in the tibiae of the P4W WT (F), conditional (F’) and conventional Fam20cD446N knock-in mice (F’’). (The yellow parentheses indicated the width of growth plates in femurs; Scale bar is 200 μm)