Fig. 8

Origin of flounder and sculpin type I AFPs from progenitor genes. A) Comparison of the corresponding genomic regions containing the HDAC5 and XYLT1 genes of starry flounder and Pacific halibut. The two alleles from an individual flounder contained either 4 or 33 AFP gene copies. B) Comparison of a single flounder Gig-2 gene and skin AFP gene. The percent identity between the homologous regions (gray shading) is indicated, with the two exons (wide) and intron (narrow) of each gene in blue or purple. The portion of the second exon containing the coding region is shaded darker. C) Comparison of the AFP gene of sculpin with the lunapark locus of a closely related fish, denoted as above, except that the lunapark exons are in orange and the lunapark is scaled 1:10 relative to the smaller AFP gene. D) Alignment of flounder, cunner and sculpin AFPs, colored as in Fig. 4A with the spacing of Thr residues (black highlighting) indicated above and asterisks indicating 100% conservation below. GenBank accession numbers are UW46952.1 (starry flounder), see Fig. 4 (cunner AFP2), and AGZ85412 (shorthorn sculpin)