Fig. 2

AFP locus in cunner compared to the syntenic location in two other wrasses. A) The cunner AFP locus on chromosome 4, showing the locations of the 11 AFP genes (cyan) relative to the GIMAPa genes (dark yellow) and GIMAPb genes (red). The arrows indicate gene orientation (all AFP genes and GIMAPa genes are transcribed left to right) and span the coding region and intron(s). Flanking genes are numbered consecutively in gray. The scale bar shows a 20-kb stretch of DNA. The GenBank accession number for the region shown is JAJGRF010000003.1, bases 6,121,000 to 6,371,000. B) Ballan wrasse locus colored as above, GenBank accession NW_018114954.1, bases 83,944 to 184,596. C) Spotty locus, colored as above, with GIMAPc genes in dark red, GenBank accession NC_048278.1, bases 5,367,202 to 5,491,088. The unrelated flanking genes, numbered sequentially, are solute carrier family 25 member 10-like (SLC25A10) and claudin-9-like (CLDN9) at the 5ʹ side and Ras-related dexamethasone-induced 1-like (RASD1), MYCBP-associated protein-like (MYCBPAP) and epsin 3-like (EPN3) at the 3ʹ side. D) Comparison of the cunner AFP11 and GIMAP-a5 genes. The percent identity between the homologous regions (gray shading) is indicated, with the two exons (wide) and intron (narrow) of each gene in cyan or dark yellow. The portion of the second exon of GIMAP-a5 that contains an Ala-rich region is shaded darker. The scale bar shows a 0.5-kb stretch of DNA