Table 3 Physico-chemical characteristics of the designed multiepitope vaccine

Number of amino acids

483

GRAVY (Grand average of hydropathicity)

− 0.421

Molecular weight (MW)

40.21 kDa

Aliphatic index

89.93

Positive residues (Arg + Lys)

56

Instability index

36.37

Theoretical isoelectric point (pI)

7.41

In-vivo evaluated half-life (E. coli)

> 14 h

Extinction coefficient (at 280 nm in H2O)

17,521 M − 1 cm − 1

In-vitro evaluated half-life (mammalian reticulocytes)

279 h

Negative residues (Asp + Glu)

62

In-vivo evaluated half-life (Yeast cells)

> 19 h