Fig. 3

Effects of laylin-KD on mitochondrial membrane potential. (A) A172 cells, transfected with siL1, siL2 or siC, were plated on 35 mm glass-based dishes. After 24 h, the culture medium was replaced with serum-free RPMI containing MT-1. Following a 30 min incubation, the cells were washed with HBSS, cultured with serum-free RPMI containing 5 µM FCCP for 30 min, and then fixed in PBS containing 4% paraformaldehyde. MT-1 was visualized using a fluorescence microscope. Scale bars: 100 μm. (B) The fluorescence intensity of MT-1 was measured using the BZ-II Analyzer Ver. 1.42. Brightness levels were quantified by calculating the sum of fluorescence intensity for all pixels. The average of brightness levels in the ‘siC, FCCP (-)’ samples, which were transfected with siC, were defined as 1.0 (n = 3 in each condition). Mean values with SD are presented. * p < 0.05, ** p < 0.01 (closed bars vs. open bars), †† p < 0.01 (vs. siC, closed bars), ^‡‡p < 0.01 (vs. siC, open bars)