Fig. 2
Effects of laylin-KD on the levels of BCL-2 family proteins in A172 cells. (A) A172 cells were transfected with control siRNA (siC) and 2 kinds of layilin siRNA (siL1 and siL2). The protein samples extracted from whole cells underwent western blotting. (B and C) Band intensities were quantified using densitometry. The intensities of layilin, BAD, BAK, BAX, BIM, BCL-2, and BCL-XL bands were normalized to β-actin bands. The average normalized intensity in the ‘siC’ samples set as 1.0. (D) The BCL-2/BAX ratios were calculated with the “siC” samples as the reference (defined as 1.0). (E) RNA extracted from the cells used for reverse transcription and subject to qPCR to estimate the mRNA levels of layilin, BAD, BCL-2, and GAPDH. Measured mRNA levels of layilin, BAD, and BCL-2 were normalized to GAPDH. In each panel, the average of the normalized or corrected values in siC-transfected samples was defined as 1.0 (n = 3 in each condition). Mean values with SD are presented. *p < 0.05, ** p < 0.01