Fig. 1

Single-cell RNA sequencing and identification of cell populations and gene signatures. A. The umap of human control and OA group chondrocyte monocyte transcriptome were visualized and colored according to the grouping obtained by undifferentiated cluster analysis. B. Distribution of patient samples in chondrocytes in control and OA groups. C. Visualization of umap colored according to cell types for human control and OA articular tissue single-cell transcriptomes. D. Dot plots showing the expression of indicated markers including SOX9, COMP, COL2A1, and ACAN for chondrocyte in control and OA articular tissue single-cell transcriptomes on the umap map. E. Dot plots showing the expression of indicated markers including HBB, HBA1, and HBA2 for hemoglobin in control and OA chondrocytes on umap map. The average cellular expression level of HBB, HBA1, and HBA2 in chondrocytes samples from different group (control, OA) is shown. Data is expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001