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Table 2 The qPCR primers designed by primer3plus tool

From: Evaluation of antibacterial and anticancer properties of secondary metabolites isolated from soil Bacillus spp focusing on two strains of Bacillus licheniformis and Bacillus siamensis

Gene name

Sequence 5’ to 3’

Product size

GAPDH

F: CGAGATCCCTCCAAAATCAA

170 bp

R: TTCACACCCATGACGAACAT

Bcl-2

F: GGATTGTGGCCTTCTTTGAG

114 bp

R: GCCGGTTCAGGTACTCAGTC

Caspase 3

F: TTTTTCAGAGGGGATCGTTG

151 bp

R: CGGCCTCCACTGGTATTTTA

Caspase 8

F: TATGGCACTGATGGACAGGA

232 bp

R: GCAGAAAGTCAGCCTCATCC

Bax

F: GACGGCCTCCTCTCCTACTT

106 bp

R: CTCAGCCCATCTTCTTCCAG