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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Optimisation of cryopreservation conditions, including storage duration and revival methods, for the viability of human primary cells

Fig. 6

Immunocytochemistry (ICC) images of HDF after 3-months of cryopreservation with different cryo mediums and revival methods: (A) direct revival and (B) indirect revival. The proliferative markers Ki67 (green) and Col-1 (red) were strongly expressed. The scale bar represents 100 μm. At the bottom is the graph showing the percentage of positive expression in ICC on (C) Ki67 staining and (D) Col-1 staining. * Indicates a significant difference compared to HPL + 10% DMSO and Cryostor with both revival methods. # Indicates a significant difference compared to HPL + 10% DMSO with the direct method. p < 0.05

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