Fig. 3

Evaluation of epithelial barrier integrity in canine colonoid-derived monolayers. A Representative phase contrast brightfield microscopy images of colonoid-derived monolayers cultured on cell culture inserts following a 24-h exposure to palmitic acid. The left panel presents the image following 24 h of exposure to the carrier control (DMSO), while the right panel depicts the image after 24 h of exposure to 30 µM of palmitic acid. Scale bar = 100 µm. B The intestinal epithelial barrier integrity was assessed through the measurement of TEER values. TEER values were recorded after treating with media containing the carrier control (DMSO) or 30 µM palmitic acid for 24 h. This assessment encompassed three biological replicates, each with three technical replicates. The error bars represent the standard error of the mean. * p < 0.05