Fig. 1
Revealing enlarged dimensions and enhanced LGR5 expression in palmitic acid-exposed 3D canine colonoids. A Representative phase contrast brightfield microscopy depicting 3D colonoids cultured in Matrigel following to 24 h of exposure to varying concentrations of palmitic acid (3, 30, 60 µM). For 0 µM, a carrier control consisting of 0.1% dimethyl sulfoxide (DMSO) in the media was used. Scale bar = 100 µm. B The organoid area (µm.2) was quantified from five randomly selected fields for each condition, encompassing a minimum of ten organoids, as assessed using ImageJ. This assessment was carried out across three biological replicates, each with three technical replicates. The error bars depict the standard error of the mean. ** p < 0.01, * p < 0.05. C The gene expression level of the stem cell marker LGR5 in colonoids exposed to varying concentrations of palmitic acid was quantified using qPCR. Each dataset was compiled from three biological replicates, each with three technical replicates. The error bars represent the standard error of the mean. ** p < 0.01