Fig. 1

DUOX1 was dependently induced by DOX in AC16 cells. Human cardiac AC16 cells were treated with 2 µmol/L of DOX to simulate and construct an in vitro model of heart failure. qRT-PCR (A) and western blot (B) detected the expression of DUOX1 at different time points. **p < 0.01. The blots were cut prior to hybridisation with antibodies during immunoblotting experiments