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Table 3 The system of PCR reaction

From: Glycyrrhizin inhibits LPS-induced inflammatory responses in goat ruminal epithelial cells in vitro

Reagents

Addition

SYBRGreen Mix

12.5 µL

Upstream primer F

0.5 µL

Downstream primer R

0.5 µL

ddH2O

9.5 µL

cDNA templet

2 µL

Total volume

25 µL