Fig. 4
From: Keratin 19 binds and regulates cytoplasmic HNRNPK mRNA targets in triple-negative breast cancer
Gene ontology identifies that K19 and HNRNPK promote p53 signaling pathway in MDA-MB-231 cells. (A) The top 10 enriched NCI-Nature pathways for mRNAs downregulated by KRT19 KO. The X axis denotes the number of genes while Y axis denotes NCI-Nature pathways terms. (B) The top 10 enriched NCI-Nature pathways for mRNAs downregulated by HNRNPK KD. The X axis denotes the number of genes while Y axis denotes NCI-Nature pathways terms. (C) The top 10 enriched NCI-Nature pathways for Cytoplasmic HNRNPK mRNAs downregulated PAR-CLIP targets after HNRNPK KD or (all mRNAs) upon overexpressing cytoplasmic HNRNPK. (D) The top 10 enriched NCI-Nature pathways for Cytoplasmic HNRNPK mRNAs downregulated PAR-CLIP targets after KRT19 KO or (all mRNAs) upon overexpressing cytoplasmic HNRNPK. (E) Cytoplasmic HNRNPK (3’UTR mRNAs) upon overexpressing cytoplasmic HNRNPK. The X axis denotes the number of genes while the Y axis denotes NCI-Nature pathways terms. Enriched GO terms among downregulated HNRNPK GO terms for top 10 pathways based on p values are shown. (F) Immunoblots from lysates of Parental MDA-MB-231 and KRT19 KO cells or (G) Parental and KRT19 KO cells stably expressing GFP or GFP-K19 with antibodies against the indicated proteins. Full-length blots/gels are presented in Supplementary Figs. 10–11