Fig. 2

K19 regulates mRNAs directly bound to HNRNPK. (A) SDS-PAGE fluorescent imaging of the crosslinked cytoplasmic HNRNPK immunoprecipitation revealed two bands migrating at ~ 60 kDa, corresponding to HNRNPK (upper band) and HNRNPJ (lower band), a putative spliced isoform of HNRNPK, in both cell lines. (B) Sequence logo from cytoplasmic HNRNPK parental and KRT19 KO cells. (C) The empirical cumulative percentages plot of the change in mRNA levels in KRT19 KO compared to parental. The analysis compares HNRNPK targets (top 10%, Red, n = 733), (top 20%, Orange, n = 1488), (top 30%, Yellow, n = 2182), (top 40%, Green, n = 2930), and non-targets (Black, n = 3145). (D) The empirical cumulative percentages plot of the change in mRNA levels in cells transiently depleted HNRNPK in both parental and KRT19 KO. The analysis compares HNRNPK targets (top 10%, Red, n = 870), (top 20%, Orange, n = 1761), (top 30%, Yellow, n = 2594), (top 40%, Green, n = 3480), and non-targets (Black, n = 1484). Targets (colored lines) binned by number of HNRNPK binding sites and non-targets (black line) with minimal gene expression of 4 fragments per kilobase of exon per million mapped fragments ((FPKM) ≥ 4) are shown