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Table 1 Primers used in the expression analyses of Bos taurus genes by RT qPCR

From: Janus Kinase 3 phosphorylation and the JAK/STAT pathway are positively modulated by follicle-stimulating hormone (FSH) in bovine granulosa cells

Gene names

Primer sequences (5’-3’)a

Accession #

AS (bp)

JAK1

Fwd: GCCCTGTGTTTTGGTATGCT; Rv: ATCTGGACAGTTCGGTGGAC

XM_024989564.1

239

JAK2

Fwd: AACGCTGAGGGGGATTATCT; Rv: ATGGTTGGGTGGATACCAGA

XM_005209981.4

156

JAK3

Fwd: GGGAGATCCAGATCCTCAAAG; Rv: GCAGATCTGTGAGGCGTAGAG

XM_010806603.3

194

TYK2

Fwd: TCCTGGAGATCTGCTTCGAT; Rv: TTCTGGGGCTGTAGCTGAGT

NM_001113764.1

208

PCNA

Fwd: AAGCCACTCCACTGTCTCCTA; Rv: TTAAGTGTGTGCTGGCATCTC

NM_001034494

207

CCND2

Fwd: GGGCAAGTTGAAATGGAACCT; Rv: TGGCAAACTTGAAGTCAGTGG

NM_001076372

155

CYP19A1

Fwd: GACCATCTGTGCTGATTCCAT; Rv: TGGTTTGAGAAGGAGAGCTTG

NM_174305.1

211

CYP11A1

Fwd: ATCATTCACCCTGAAGACGTG; Rv: GCTGACGAAGTCCTGAGACAC

NM_176644.2

240

RPL19

Fwd: GACCAATGAAATCGCCAATGC; Rv: ACCTATACCCATATGCCTGCC

NM_001040516

154

  1. AS Amplicon size (base pairs), Fwd Forward primer, Rv Reverse primer
  2. aAll primers were designed and validated by the authors. Each primer was used at a final concentration of 600 nM
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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