Fig. 4

CIRP overexpression increased EGF level on the glial cells. Double-labeling immunofluorescence staining and confocal microscopy to EGF (green A), CIRP (red B), DAPI staining (blue C)and merge (D). Western blot analysis of CIRP and EGF, and the relative expression of CIRP and EGF E. CON: normal control group, MOCK: negative control group, OE: overexpression of CIRP group. Experiments were repeated three times. Bars indicated means ± SD. *p<0.01