Fig. 3

Characterization of the purified exosome. MSC-derived exosomes were dispersed in phosphate buffer saline and then measured at 30 μg/mL concentration (the figure shows a representative line plot of one sample. All samples were assessed in duplicate). Exosome size distribution by dynamic light scattering (DLS) is shown (A). Morphology of UC-MSC-derived exosomes under a transmission electron microscope (TEM, 20000 x magnification) (B). western blot analysis of isolated MSCs-derived exosomes. Lane 2 and 3 are shown two different concentrations of 15.0 and 150 μg/ml, respectively. Lane 1 is a PBS, as a negative control (C). CD63 and CD8 surface marker expression of isolated exosomes by flow cytometry (D)