Fig. 4
Effect of TMZ and a TRPA1 agonist inhibitor on intracellular Ca2+ levels. The levels of intracellular Ca2+ were increased by exposure to TMZ and PF-4840154 cotreatment, as determined by fluorescence microscopy using a Flexstation®2 using a Fluo-4 A.M. in U251 cells (A) and SHG-44 cells (B). Representative microscopy images are shown (100 x) with mean values and data from individual plate reader experiments reported graphically below. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001