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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: MicroRNA-145-5p modulates Krüppel-like factor 5 and inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma

Fig. 4

MiR-145-5p regulates KLF5 protein expression. a Western blot analysis of KLF5 levels in NPC-TW03 and NPC-TW04 cells post-transfection with scramble control or miR-145-5p mimics, were probes with anti KLF5 and specific antibodies for each compartments corresponding to anti β-actin. b Quantification of KLF5 expression are the average of three independent experiments, using the Image J software using β-actin as a reference. c MiR-145-5p down-regulates KLF5 mRNA expression after scramble control or miR-145-5p mimics transfection in NPC-TW03 and NPC-TW04 cells. d Complementarity between the seed sequence of hsa-miR-145-5p and the targeted sequence in the 3'-UTR of KLF5 mRNA predicted by TargetScan. e Relative luciferase activity of wild-type (WT) and mutated KLF5 3'-UTR luciferase reporters in NPC-TW03 and NPC-TW04 cells after miR-145-5p mimics or scramble control co-transfection. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, respectively

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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