Fig. 3

Spheroid confocal fluorescence microscopy. A Spheroids were stained with Live/Dead® Viability/Cytotoxicity kit reagents to detect live (green) or dead (red) cells of Aire^WT or Aire^−/− spheroids B Quantitative analysis of the fluorescence signal for live or dead cells comparing Aire^WT and Aire^−/− spheroids. Time-point values correspond to mean and ± SD from 20 spheroids analyzed. The MFI of the channels green and red was measured and compared through paired student t-test (p-value = *** 0,0008; ns 0,8097- 0,8196; * 0,0237 respectively). ns: no significant difference