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Table 2 Plasmids used in this study

From: Exportin Crm1 is important for Swi6 nuclear shuttling and MBF transcription activation in Saccharomyces cerevisiae

Plasmid number

Genotype

Source

pRS315

CEN LEU2

[65]

pKBB353

CEN URA3 SWI61–181-GFP

This study

pKBB354

CEN URA3 SWI61–273-GFP

This study

pKBB416

CEN URA3 SWI61–273ΔNES-GFP

This study

pKBB484

CEN URA3 GAL1::SWI6-GFP

This study

pGAL::CCA1-GFP

CEN URA3 GAL1::CCA1-GFP

[46]

pGAL::H2B-GFP

CEN URA3 GAL1::H2B-GFP

[46]

pAC1202

CEN TRP1 SWI6-GFP

[43]

pAC1571

CEN LEU2 SWI6-GFP

[43]

pKBB418

CEN TRP1 SWI6ΔNES-GFP

This study

pKBB441

CEN LEU2 SWI6ΔNES-GFP

This study

pOBD-2

CEN TRP1 GAL4-BD

[56]

pOAD

CEN LEU2 GAL4-AD

[56]

pKBB486

CEN TRP1 CRM1-BD

This study

pKBB491

CEN LEU2 SWI6-AD

This study

pBA251

CEN URA3 4X SCB::LacZ

[4]

pBA487

CEN URA3 4X MCB::LacZ

[4]

pLDB235

CEN URA3 GAL1

L. Davis

pLDB350

CEN URA3 GFP

[66]

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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