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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Spinoculation and retronectin highly enhance the gene transduction efficiency of Mucin-1-specific chimeric antigen receptor (CAR) in human primary T cells

Fig. 1

The comparison of transfection efficiency between PEI, lipofection and calcium phosphate methods. A) Co-transfection of pLJM1-EGFP:pRSV-Rev:pMDLg/pRRE:pMD2.G plasmids into HEK-293 T cells. Fluorescence microscopy of GFP indicates higher expression levels of GFP in PEI and lypofectamine transfection methods than calcium phosphate method. B) Transfection rate of mentioned methods. C) The effect of transfection methods on viability of HEK-293 T cell. The scale bar represents 100 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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