Fig. 3
From: KDM6A mutations promote acute cytoplasmic DNA release, DNA damage response and mitosis defects
RBBP5 and NPM1 interaction analysis. Top section: KDM6A variants used in the interaction study. Mid/Bottom section: IP of KDM6A variants with RBBP5 and NMP1 in SW-1710 and T-24 (n = 2–7). The cleared cell lysates (left section) indicates GFP, RBBP5 and NMP1 protein levels after transfection of KDM6A WT, variants and the eGFP control. For SW-1710 (right section, mid) and T-24 (bottom), IP was used to pull RBBP5 with eGFP-KDM6A WT, D336G, T726K, ΔTPR, ΔJmjC, TPR, JmjC and the control eGFP. In SW-1710, NPM1 is slightly enriched in the IP in the eGFP-KDM6A ΔTPR and ΔJmjC variants (white stars), whereas in T-24, NPM1 is enriched in eGFP-KDM6A T726K variant (n = 1). The TPR and RBBP5 signals are separately shown in Figure S5, as well as controls and raw Western Blot images