Fig. 6

Quadruple mutant attenuated PKC effect on OAT1 transport function. OAT1-expressing COS-7 cells were transfected with either wild type (WT) or quadruple mutant (Quad) of Nedd4–2. Transfected cells were then treated with 5 μM staurosporine (St) for 1 h. 4-min uptake of [³H]-para-aminohippuric acid (PAH, 20 μM) was then measured at room temperature. Each data point represents OAT1-mediated transport only, after subtracting uptake values from cells transfected with empty vector. Uptake value in each group is expressed as a percentage of the uptake value measured in control cells. Values are mean ± S.D. (n = 4). *p < 0.05