Fig. 5
From: Intracellular targeting of Cisd2/Miner1 to the endoplasmic reticulum
Expression of CD1b-tagged Cisd2 alters the morphology of the endoplasmic reticulum. a Cells transfected with CD1b-M1 were fixed, sectioned, labelled with anti-CD1b antibodies, revealed with a gold-coupled secondary antibody and observed by electron microscopy. b Cells transfected with CD1b-M1 were fixed, embedded in Epon resin, stained and sectioned. The nucleus is indicated (N), as well as the location of the nuclear envelope (arrowheads). Scale bar: 1 μm. In the two instances shown, the endoplasmic reticulum collapsed on the nuclear envelope with which it connected in multiple points