Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities

Table 4 mRNA expression of myogenesis-associated genes (least square means ± standard errors) after 72 h of proliferation

Gene	Temperature (T)				Pool (P)		T P	P P	T × P P
Gene	35 °C	37 °C	39 °C	41 °C	5	20	T P	P P	T × P P
Myogenic transcription factors
PAX7	0.92 ± 0.06 ^a	0.76 ± 0.06 ^ab	0.88 ± 0.06 ^ab	0.66 ± 0.06 ^b	0.91 ± 0.04	0.69 ± 0.04	0.022	< 0.001	0.846
MYF5	0.96 ± 0.06 ^a	0.71 ± 0.06 ^a	0.45 ± 0.06 ^b	0.38 ± 0.06 ^b	0.78 ± 0.04	0.47 ± 0.04	< 0.001	< 0.001	0.426
MRF4	1.87 ± 0.19	1.87 ± 0.19	1.67 ± 0.21	0.88 ± 0.29	1.05 ± 0.15	2.09 ± 0.17	0.061	< 0.001	0.291
Growth factors and growth factor receptors
MSTN	0.85 ± 0.04 ^a	0.69 ± 0.04 ^b	0.42 ± 0.04 ^c	0.37 ± 0.04 ^c	0.70 ± 0.03	0.46 ± 0.03	< 0.001	< 0.001	0.233
IGF2	0.67 ± 0.07 ^b	0.71 ± 0.07 ^b	0.74 ± 0.07 ^ab	1.02 ± 0.07 ^a	1.01 ± 0.05	0.55 ± 0.05	0.015	< 0.001	0.195
EGF	0.79 ± 0.15 ^b	0.71 ± 0.15 ^b	1.09 ± 0.15 ^b	2.42 ± 0.15 ^a	1.27 ± 0.10	1.24 ± 0.10	< 0.001	0.828	0.384
IGF1R	0.89 ± 0.08 ^ab	0.65 ± 0.08 ^b	1.00 ± 0.08 ^a	0.83 ± 0.08 ^ab	0.71 ± 0.06	0.96 ± 0.06	0.043	0.006	0.082
EGFR	1.22 ± 0.10 ^ab	0.88 ± 0.10 ^b	1.36 ± 0.10 ^a	1.40 ± 0.10 ^a	1.06 ± 0.07	1.37 ± 0.07	0.009	0.008	0.165

The mRNA expression data are expressed as arbitrary units after normalization with the endogenous reference gene RN18S
^{a, b, c} Labeled least square means within a row with different letters differ (P < 0.05)

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