Fig. 5

Glycolysis and ATP synthesis of cell lines. ECAR measured using Agilent Seahorse XFe96 analyzer of 20,000 live cells/well (A), and calculated glycolysis (B) and glycolytic capacity (C) are shown. The mean values were compared between the parent HEK293 and the mutant HEK293ΔSDHD. ATP synthesis of parent and mutant cells measured using Mitochondrial ToxGlo Assay (D) is shown. The measurements are expressed as total luminescence per 3500 or 10,000 cells. Error bars represent the SE of the mean