Fig. 2

The effect of E343 mutation on cell migration. Cell migration was evaluated by Transwell assay, and the cells migrated through the membrane were fixed in formaldehyde and stained with crystal violet, then imaged using the microscope (a) and quantified the cell number using ImageJ software (b). Cell migration was evaluated by wound healing assay (c) and the migration rate was quantified (d). Scale bar: 100 μm, *p < 0 .05, **p < 0 .01, ***p < 0 .001