Table 1 List of gene primers, annealing temperature and amplicon size for Sus scrofa domesticus gene amplification used in RT-PCR protocols
From: A novel method for isolation and culture of primary swine gastric epithelial cells
Target Gene | RefSeq. | Sense 5′-3’ | Antisense 5′-3’ | Tm (°C) | Size (bp) |
|---|---|---|---|---|---|
MUC1 | XM_021089730.1 | GACGGGCTTCTGGGACTCTTTTA | TGCTCATAGGGGTTCCGTTTGGTA | 58 | 437 |
MUC20 | NM_001113440.1 | GACCTCACTGACCCCACAGT | CTGATGTACGTGGGAACCT | 58 | 341 |
ACTB | AY550069.1 | GGCACCACACCTTCTACAAC | GAGTCCATCACGATGCCAGT | 58 | 208 |