Fig. 7

Luciferase activity of the site-directed mutation plasmid pGL3-mut-ga. The distance between pGL3-hsf1-r3 and pGL3-hsf1-r4 contains a sole GATA-1 binding site (ATCTGTTCCC) in the promoter of HdHSF1. When TG was mutated into CA, the sequence became ATCCATTCCC, and the mutant recombinant plasmid was named pGL3-mut-ga. The pGL3-basic plasmid was served as a negative control. (The different letters on the error bars represent significant differences, p < 0.05). Luc: luciferase expression plasmids