Fig. 1

Expression analysis of the heat shock-related genes when the HdHSF1 was inhibited by dsRNA in hemocytes. a. the mRNA expression level of the HdHSF1 gene in the HdHSF1 RNAi group was significantly downregulated compared with the GFP RNAi group and the blank control group (p < 0.05). b. the mRNA expression levels of 8 heat shock-related genes after the interference of HdHSF1. The X-axis represents treatment conditions and different target heat shock responsive transcripts. Y-axis represents the mRNA expression level of different genes. Six biological replicates were tested, and each sample was assayed in triplication. A significant difference between the experimental group and the control group was indicated by a (*) at p < 0.05. Control: blank control group. GFP-RNAi: group in which green fluorescent protein (GFP) gene was inhibited by dsRNA. HdHSF1-RNAi: group in which HdHSF1 was inhibited by dsRNA