Fig. 3
From: MazEF-rifampicin interaction suggests a mechanism for rifampicin induced inhibition of persisters
E. coli death phase does not show apoptotic hall marks. Annexin V/PI was used to determine whether apoptotic like death occurred during the death phase of the bacterial growth curve. E. coli at different time points of the growth curve showed no change in the Annexin V/PI labelled cells, whereas, Kanamycin treated showed increased number of Annexin V/PI labelled cells (Panels a). DNA fragmentation pattern (Panel b) and membrane depolarization (Panel c) are shown for cells at different stages of growth in a batch culture. All figures are representative images of three independent experiments