Fig. 5

Determination of optimal number of reference genes needed for normalization (geNorm) for both cultures using pairwise V_n/n + 1 analysis. The recommended cutoff is the lowest V_n/n + 1 below the threshold of 0.15. In the present study, both the cultures had V_2/3 less than 0.15, indicating addition of a third reference gene would not affect normalization results