Fig. 6
From: Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles
APP endolysosomal localization is altered in the absence of Alix and Syntenin-1. a APP immunofluorescent staining was performed on control HEK293 cells expressing APPswe or following induction of Alix (shAlix) or Syntenin-1 (shSyntenin-1) shRNA knockdown. To assess subcellular compartment localization of APPswe in the absence of Alix and Syntenin, GFP-tagged APPswe was introduced into cells. Cells were stained with (b) DHPE or (c) Lysotracker before live-cell imaging on a Zeiss LSM 880 confocal microscope. Images are representative across at least three independent experiments. Scale bar = 20 μm. Pearson’s Correlation Coefficient (PCC) calculated from ≥10 cells. One-way ANOVA: **, p < 0.01. *, p < 0.05