Fig. 5

a β-catenin protein expression in the whole cell, nucleus and cytoplasm of SW1353 cells at 48 h after over-expression of HOTAIR by retrovirus infection compared to control cells. Histone-H3 was used as a nuclear protein control and GAPDH as a cytoplasmic protein control. b Expression levels of c-Myc, ZEB1 and SNAIL as downstream targets of β-catenin signalling in SW1353 cells over-expressing HOTAIR and controls. c Schematic of the proposed mechanism by which HOTAIR contributes to cartilage degradation in OA