Table 1 Plasmid constructs
From: Klotho is regulated by transcription factor Sp1 in renal tubular epithelial cells
Name | Sequence |
|---|---|
SP1 forward primer | 5′-TCACTATAGGGAGACCCAAGCTTATGGATGAAATGACAGCTGT-3′ |
SP1 reverse primer | 5′-CCGAGCTCGGTACCAAGCTTTCAGAAGCCATTGCCACTGA-3’ |
KL5 forward primer | 5′-TCTGCGATCTAAGTAAGCTTCTCCGAGTGGGAGAAAAGT-3’ |
KL5 reverse primer | 5′-AGTACCGGAATGCCAAGCTTGCTGCGCGGGAGCCAGGCT-3’ |
KL4 forward primer | 5′-TCTGCGATCTAAGTAAGCTTCGGGAGCTGGGAGAAACAG-3’ |
KL4 reverse primer | 5′-AGTACCGGAATGCCAAGCTTGCTGCGCGGGAGCCAGGCT-3’ |
KL3 forward primer | 5′-TCTGCGATCTAAGTAAGCTTTCCCGGGCACCCCTCGCCCT-3’ |
KL3 reverse primer | 5′-AGTACCGGAATGCCAAGCTTGCTGCGCGGGAGCCAGGCT-3’ |
KL2 forward primer | 5′-TCTGCGATCTAAGTAAGCTTTCGCAGGTAATTATTGCCA-3’ |
KL2 reverse primer | 5′-AGTACCGGAATGCCAAGCTTGCTGCGCGGGAGCCAGGCT-3′ |
KL1 forward primer | 5′-TCTGCGATCTAAGTAAGCTTCGGGCATAAAGGGGCGCGG-3’ |
KL1 reverse primer | 5′-AGTACCGGAATGCCAAGCTTGCTGCGCGGGAGCCAGGCT-3’ |